6, Rue Ibn Ennafis - Z.I. Lac 3 Tunisie
Tél. : 71 182 500 - Fax : 71 182 250
www.biomaghreb.com
IVD
FT En 07 Date of updating : 10/ 2020 Version B
IN VITRO DIAGNOSTIC USE
Manufacturer Use by In Vitro Diagnostic Temperature
Limitation
Catalogue number See insert Store away from light Sufcient
for < n > essais
Batch number
INORGANIC PHOSPHORUS
U.V Method
Reagent for the quantitative determination
of inorganic phosphorus in human plasma and urine
CLINICAL SIGNIFICANCE
Phosphorus is an essential mineral for many cellular reactions, particularly for bone formation and
the cell’s energy mechanisms. Decreased phosphorus levels can cause hypervitaminosis D, hyper-
thyroidism, or kidney dysfunction. Hyperphosphatemia is often associated with liver or kidney dys-
function or bone metastases. Clinical diagnosis should be made taking into account clinical and
laboratory data.
PRINCIPLE
Inorganic phosphorus is dosed according to the following reaction
Phosphore
Ammonium
Molybdate
+
Sulfuric
Acid
phospho-molybdate
Complex
The absorbance of this complex at 340 nm is proportional to the phosphorus concentration.
REAGENT COMPOSITION
Reagent 1
Sulfuric acid
Ammonium Molybdate
Detergent OS
200 mmol/l
0.40 mmol/l
Reagent 2 Standard
5 mg/dl
50 mg/l
1.61 mmol/l
SAFETY CAUTIONS
Biomaghreb reagents are intended for use by qualified personnel for in vitro use (do not pipette by
mouth).
• Consult the current MSDS available on request or on www.biomaghreb.com.
• Check the integrity of the reagents before use.
• Disposal of waste: comply with the legislation in force.
For safety reasons, treat any specimen or reagent of biological origin as potentially infectious.
Observe the applicable legislation.
REAGENT PREPARATION
Reagents are ready to use.
SAMPLE PREPARATION
Serum, plasma (not hemolyzed).
Urine diluted 1/10 with distilled water.
PRESERVATION AND STABILITY
Until the expiry date indicated on the label of the box .
ADDITIONAL EQUIPMENT
• Equipement de base du laboratoire d’analyses médicales ;
• Spectrophotomètre ou Analyseur de biochimie clinique.
LIMITS
If the sample is lipemic, make a blank by mixing 10 µl of sample with 1 ml of 9g/l NaCl solution and
read off the optical density at 340 nm.
The standard is an aqueous solution.
It is preferable to use standards of serum origin in particular, for reasons of viscosity on automatic
analysers.
QUALITY CONTROL
External quality control program.
It is recommended to control in the following cases:
• At least one test per series.
• Change of reagent bottle.
• After maintenance work on the analyzer.
If a control value is outside the confidence limits, repeat the procedure using the same control.
Use normal and pathological control sera.
CALIBRATION
The standard of the kit (Reagent 2) or any calibrator connected to a method or reference material.
The frequency of calibration depends on analyzer performance and reagent storage conditions.
Recalibration is recommended in the following cases:
1. Changing the reagent lot ;
2. After maintenance work on the analyzer; and
3. Control values are outside the confidence limits.
LINEARITY
The method is linear up to 200 mg/l (20 mg/dl – 6.46 mmol/l).
PROCEDURE
Wavelength: 340 nm
Temperature : 20°C - 25°C
Tank : 1 cm thick
Adjust the spectrophotometer zero on the reagent blank.
Blank Standard Sample
Standard
- - 10 µl - -
Sample
- - - - 10 µl
Working Solution
1 ml 1 ml 1 ml
Mix and read optical densities after a 5-minute incubation at room
temperature. Staining is stable 30 minutes.
CALCULATION
Phosphorus =
OD. Sample
OD Standard
x n
n = Standard Value
n = 5 mg/dl ;
n = 50 mg/l ;
n = 1.61 mmol/l.
REFERENCES VALUES
Adults
2.5 - 5 mg/dl
25 - 50 mg/l
0.81 - 1.61 mmol/l
Children
4 - 7 mg/dl
40 - 70 mg/l
1.29 - 2.26 mmol/l
Urine
16.5 - 48.5 mmol/24h
0.5 - 1.5 g/24h
REFERENCES
Daly J.A, Ertingshaussen G., Clin. Chem., 18, 263 (1972).
