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IVD
APTT
Activated Prothrombin time
FT En 32 Date of updating : 10/ 2020 Version B
IN VITRO DIAGNOSTIC USE
Manufacturer Use by In Vitro Diagnostic Temperature
Limitation
Catalogue number See insert Store away from light Sufcient
for < n > essais
Batch number
CLINICAL SIGNIFICANCE
Activated partial thromboplastin time (APTT) test is used for diverse purposes of preoperative scree-
ning, screening for coagulation factors deficiency, screening for various types of coagulation inhi-
bitors (e.g. lupus anticoagulants) and for controlling heparin therapy. The APTT determination is a
measure of the intrinsic and the final common pathway of the coagulation cascade integrity
PRINCIPLE
Cephalin, an extract of cerebral lipids, used as a substitute for platelet factors, provides an overall
measure of plasma factor deficits in endogenous thromboplastin formation.
Kaolin is used as a contact phase factor activator.
REAGENT COMPOSITION
Cephalin Kaolin (R1): lyophilisate
Calcium Chloride 0,025 (
REF
33017); ready to use
REAGENT PREPARATION
Cephalin Kaolin: Dissolve the bottle with 3 ml of distilled water. Leave for 15 minutes at room tempe-
rature (20-25°C). Shake frequently before use.
SAFETY CAUTIONS
Biomaghreb reagents are intended for qualified personnel, for in vitro use (do not pipette with the
mouth).
• Consult the current MSDS available on request or on www.biomaghreb.com;
• Check the integrity of the reagents before use; and
• Disposal of waste: comply with the legislation in force.
For safety reasons, treat any specimen or reagent of biological origin as potentially infectious. Res-
pect the legislation in force.
SAMPLE PREPARATION
Collect blood on sodium citrate: 9 volumes of blood to 1 volume of anticoagulant (3.8% sodium
citrate solution crystallized at 5.5 H2O), centrifuge at 4000 rpm (2500g) for at least 20 minutes.
The test must be carried out within a period not exceeding 4 hours after sampling.
At the same time, two tubes of control blood will be taken and treated in the same way as the patient’s
blood.
PRESERVATION AND STABILITY
• Before opening : (lyophilisate) between 2-8°C, until the deadline indicated on the box.
• After opening : (Reconstituted) : 4 weeks between 2-8°C.
PROCEDURE
All determinations must be made in duplicate.
In a tube placed in a water bath at 37°C, put successively:
Plasma sample (patient or control) 100 µl
Céphaline-Kaolin 100 µl
After incubation for 3 minutes at 37°C,
Add by simultaneously triggering the stopwatch.
Ca Cl
2
(0.025 M) pre-incubated at 37°C 100 µI
Note the clotting time.
ADDITIONAL EQUIPMENT
• Basic equipment of the medical analysis laboratory;
• Automatic or semi-automatic coagulation analyser.
RESULTATS
Note the coagulation times of the control and the tested plasmas.
The normal time is between 30 and 40 seconds. An increase of more than 8 seconds compared to the
controls should prompt further exploration.
During a preoperative examination, vascular or platelet damage, a normal Cephalin Kaolin time seems
to protect against uncontrollable bleeding.
The therapeutic zone of the Cephalin-Kaolin time under heparin is generally between 1.5 and 2 times
the control time.
