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Reagents  SUBSTRATS
CHOLESTEROL
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PRESENTATION
Ref. 20111, (360 Tests)
R1: 3 x 120
ml
R2: 3 flacons (Lyoph)
R3: 1 x 5 ml
Ref. 20115,(600 Tests)
R1 : 6 x100 ml
R2 : 6 flacons (Lyoph)
R3 : 2 x 5 ml
Ref. 20112(120 Tests)
R1 : 4 x 30 ml
R2 : 4 flacons (Lyoph)
R3 : 1x 4ml

PRINCIPLE :

The cholesterol is determined after enzymatic hydrolysis and oxidation. The indicator quinoneimine is formed from hydrogen peroxide and 4-aminoantipyrine in the presence of phenol and peroxidase :

The quantity of this red dye quinonimine formed is proportional to the cholesterol concentration.

REAGENTS :

Reagent 1 :
Buffer solution

Pipes pH 6.9
Phenol

90 mmol/l
26 mmol/l

Reagent 2 :Cholesterol oxidase
Peroxidase
Cholesterol esterase
4-Aminophenazone
300 U/l
1250 U/l
300 U/l
0.4 mmol/l
Reagent 3 :
Standard
 200 mg/dl
2 g/l
5.17 mmol/l

 PREPARATION AND STABILITY :

Reconstitute one vial of reagent 2 with the appropriate volume of buffer/reagent
This working reagent is stable :
- 4 months at 2 - 8¬įC
- 1 month at 20 - 25¬įC

 SAMPLES :

Serum heparinized Plasma.

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