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Reagents  SUBSTRATS
URIC ACID
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PRESENTATION
Ref. 20091, (375 Tests)
R1 : 3 x 125 ml
R : 3 flacons (Iyoph)
R3 : 1 x 6 ml
Ref 20092, (120 Tests)
Rl : 4 x 30 ml
R2 : 4 flacons (Iyoph)
R3 : 1 x 4 ml

Ref 20095, (240 Tests)
R1 : 2 x 120 ml
R2 : 2 flacons (Iyoph)
R3 : 1 x 5ml

PRINCIPLE :

Uric acid is oxidized by uricase to allantoine and hydrogen peroxide, according to the following equation :

REAGENTS :

Reagent 1
Buffer solution

Phosphate buffer pH 7.4

2-4 DHBS

50mmol/l


4 mmol/l

Reagent 2 :
vial of enzymes
Uricase
Peroxidase
4-Aminophenazone.
70 U/l
660 U/l
1 mmol/l
Reagent 3 :
Standard
Uric Acid6 mg/dl
60 mg/l
357µmol/l

PREPARATION AND STABILITY :

- Reconstitute one vial reagent 2 with 30 ml of buffer/reagent 1 (Ref 20092).
- Reconstitute one vial reagent 2 with 1 bottle (125 ml) of buffer/reagent 1 (Ref 20091).
- This working reagent is stable :
- 3 weeks at 2-8°C
- 7 days at 20-25°C

 SAMPLES :

Serum, plasma collected on heparin. Urine diluted 1 to 10 in distilled water.

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