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Reagents  ENZYMES
ALAT - GPT
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PRESENTATION
Ref. 20046, (60 Tests)
R1: 1 x 65 ml
R2: 20 x 3 ml(Iyoph)
Ref 20047, (100 Tests)
R1: 1 x 110 ml
R2: 10 x 10 ml (Iyoph)
Ref. 20040, (450 Tests)
R1: 9 x 50 ml
R2: 9 x 50 ml (Iyoph)

PRINCIPLE :

Kinetic determination of Alanine Amino transferase activity.
Method recommended by the IFCC.

The rate of NADH consumption is determined photometrically and is directly proportional to the GPT activity in the sample.
GPT: Glutamic pyruvate transamise.
LDH: Lactate dehydrogenas.

REAGENTS :

Reagent 1
Buffer reagent

Tris buffer PH7.5
L-Alanine

100 mmol/l

500 mmol/l

Reagent 2 : SubstratNADH

LDH
Oxoglutarate
0.18 mmo/l
1200 Ull
15 mmol/l

PREPARATION AND STABILITY :

Reconstitute one via of reagent 2 with the appropriate volume of buffer reagent 1
This working reagent is stable:
- 7 days at 2-8°C.
- 24 hours at 20-25°C.

 SAMPLES :

Serum, heparinized plasma free of hemolysis.

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