PRESENTATION Ref.20042, (60 Tests) R1: 1 x 65 ml R2: 20 x 3 ml(Iyoph) Ref 20043, (100 Tests) R1: 1 x 110 ml R2: 10 x 10 ml (Iyoph) Ref. 20050, (450 tests) R1 :9x50ml R2: 9 x 50 ml (Iyoph)

PRINCIPLE : Kinetic determination of Aspartate amino transferase activity. Method recommended by the IFCC  The rate of NADH consumption is determined photometically and is directly proportional to the GOT activity in the sample. GOT: Glutamic oxaloactate transaminase MDH: Malate dehydrogenase

REACTIFS :

Reagent 1 Buffer reagent	Tris buffer PH 7.8 L- aspartate	C80 mmol/l 200 mmol/l
Reagent 2 : Substrat	NADH LDH MDH Oxoglutarate	0.18 mmo/l 800 U/l 600 U/l 12 mmol/l

PREPARATION AND STABILITY : Reconstituate one vial of Reagent 2 with the appropriate volume of buffer/Reagent 1. This working reagent is stable : - 7 days at 2-8°C. - 24 hours at 20-25°C.

 SAMPLES : Serum heparinized plasma free of  heamolysis.