 PRESENTATION
Ref.20042,
(60 Tests)
R1: 1 x 65 ml
R2: 20 x 3 ml(Iyoph) | Ref 20043,
(100 Tests)
R1: 1 x 110 ml
R2: 10 x 10 ml (Iyoph) | Ref. 20050,
(450 tests)
R1 :9x50ml
R2: 9 x 50 ml (Iyoph) |
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PRINCIPLE :
Kinetic determination of Aspartate amino transferase activity.
Method recommended by the IFCCÂ 
The rate of NADH consumption is determined photometically and is directly proportional to the GOT activity in the sample.
GOT: Glutamic oxaloactate transaminase
MDH: Malate dehydrogenase |
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REACTIFS :
Reagent 1
Buffer reagent | Tris buffer PH 7.8
L- aspartate | C80 mmol/l
200 mmol/l | | Reagent 2 : Substrat | NADH
LDH
MDH
Oxoglutarate | 0.18 mmo/l
800 U/l
600 U/l
12 mmol/l | |
| PREPARATIONÂ AND STABILITY :
Reconstituate one vial of Reagent 2 with the appropriate volume of buffer/Reagent 1.
This working reagent is stable :
- 7 days at 2-8°C.
- 24 hours at 20-25°C. |
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 SAMPLES :
Serum heparinized plasma free of  heamolysis. |
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